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TBC1D15 Polyclonal antibody

TBC1D15 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17252-1-AP

Print datasheet

Synonyms

TBC1 domain family member 15, Rab7-GAP, Rab7 GAP, GTPase-activating protein RAB7, GTPase activating protein RAB7

验证数据展示

  • WB analysis using 17252-1-AP

    WB analysis using 17252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 17252-1-AP

    WB analysis using 17252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17252-1-AP

    IF Staining of HeLa using 17252-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBC1D15 antibody (17252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, A431 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

17252-1-AP targets TBC1D15 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TBC1D15 fusion protein Ag10625 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TBC1 domain family, member 15
Synonyms TBC1 domain family member 15, Rab7-GAP, Rab7 GAP, GTPase-activating protein RAB7, GTPase activating protein RAB7
Calculated Molecular Weight 691 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC028352
Gene Symbol TBC1D15
Gene ID (NCBI) 64786
RRIDAB_2878370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D15 is a highly conserved protein belonging to the TBC domain family and has similar functions to Rab-GAP. TBC1D15 is ubiquitously expressed and localized predominantly to the cytosol. TBC1D15 is a protein that contains a conserved Tre2/Bub2/Cdc16 (TBC) domain. TBC1D15 can serve as a mitochondrial Rab GTPase-activating protein (Rab-GAP) and influence autophagosome biogenesis and morphology downstream of Parkin activation. TBC1D15 can also control stem cell self-renewal when coupled with the Numb-p53 complex.

实验方案

Product Specific Protocols
WB protocol for TBC1D15 antibody 17252-1-APDownload protocol
IF protocol for TBC1D15 antibody 17252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Death Dis

SENP3-FIS1 axis promotes mitophagy and cell survival under hypoxia

Authors - Alice Zhao
View Article