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验证数据展示

  • WB analysis using 17269-1-AP

    WB analysis using 17269-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17269-1-AP (COQ10B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 17269-1-AP

    IF Staining of A431 using 17269-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COQ10B antibody (17269-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

COQ10B Polyclonal antibody

COQ10B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17269-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Coenzyme Q-binding protein COQ10 homolog B, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17269-1-AP targets COQ10B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 COQ10B fusion protein Ag11024 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coenzyme Q10 homolog B (S. cerevisiae)
别名 Coenzyme Q-binding protein COQ10 homolog B, mitochondrial
计算分子量 238 aa, 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC022453
基因名称 COQ10B
Gene ID (NCBI) 80219
RRIDAB_3669265
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H8M1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COQ10B (coenzyme Q10, homolog B) is an anti-apoptosis enzyme stored in the mitochondria of cells and belongs to the COQ10 family. COQ10B is an important biological cofactor, which can increase the concentration of mitochondria in the brain and play a neuroprotective role (PMID: 35613207). COQ10B is an isoform of COQ10 and has been detected in chronic stress-associated intestinal barrier dysfunction, acute pelvic inflammatory illness, moyamoya disease, and pelvic inflammatory disease (PMID: 35911165). The calculated molecular mass of COQ10B is 27 kDa.

实验方案

Product Specific Protocols
WB protocol for COQ10B antibody 17269-1-APDownload protocol
IF protocol for COQ10B antibody 17269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols