验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IF | See 3 publications below |
产品信息
17521-1-AP targets ACTA1-Specific in WB, IHC, FC (Intra), ELISA, IF applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin, alpha 1, skeletal muscle |
| 别名 | ACTA1, Alpha Actin, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form |
| 计算分子量 | 42 kDa |
| 观测分子量 | 39-42 kDa |
| GenBank蛋白编号 | NM_001100 |
| 基因名称 | ACTA1 |
| Gene ID (NCBI) | 58 |
| RRID | AB_2273608 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P68133 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| IHC protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| FC protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome. | ||
Cancer Lett Cancer-associated fibroblasts-derived exosomal miR-17-5p promotes colorectal cancer aggressive phenotype by initiating a RUNX3/MYC/TGF-β1 positive feedback loop. | ||
Mol Ther Nucleic Acids Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis | ||
Int J Mol Sci Neddylation Regulates Class IIa and III Histone Deacetylases to Mediate Myoblast Differentiation | ||
Aging (Albany NY) Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats. | ||
iScience A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart |
