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WDFY4 Polyclonal antibody

WDFY4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17558-1-AP

Print datasheet

Synonyms

C10orf64, KIAA1607, WDFY family member 4, WDFY4

验证数据展示

  • IHC staining of human tonsillitis using 17558-1-AP

    IHC staining of human tonsillitis using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 17558-1-AP

    IHC staining of human tonsillitis using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17558-1-AP targets WDFY4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen WDFY4 fusion protein Ag11720 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WDFY family member 4
Synonyms C10orf64, KIAA1607, WDFY family member 4, WDFY4
Calculated Molecular Weight 354 kDa
Observed Molecular Weight ~70 kDa
GenBank Accession NumberBC032420
Gene Symbol WDFY4
Gene ID (NCBI) 57705
RRIDAB_2288447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZS81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDFY4, also named as C10orf64 and KIAA1607, is a huge protein with unknown functions. WDFY4 is expressed predominantly in immune tissues such as lymph nodes, the spleen, the thymus and the tonsils. According to recent genome-wide association studies (GWAS), WDFY4 was introduced as novel predisposing genes for SLE (Systemic Lupus Erythematosus) which is a heterogeneous complex relapsing-remitting autoimmune disease. This antibody detects the C-terminal of WDFY4. The gene WDFY4 has some isoforms, 354 kDa, 73 kDa, 115 kDa, 353 kDa, 325 kDa( from UNIPROT) and 1992aa, 222 kDa; 3224aa, 358kDa; 624aa, 69 kDa; 1270aa, 143 kDa; 1887aa, 211 kDa (From NCBI).

实验方案

Product Specific Protocols
IHC protocol for WDFY4 antibody 17558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols