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验证数据展示

  • WB analysis of HeLa using 18001-1-AP

    WB analysis of HeLa using 18001-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18001-1-AP

    IHC staining of human kidney using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 18001-1-AP

    IHC staining of human kidney using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

PPCS Polyclonal antibody

PPCS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18001-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

COAB, PPC synthetase, PPCS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

18001-1-AP targets PPCS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PPCS fusion protein Ag12491 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphopantothenoylcysteine synthetase
别名 COAB, PPC synthetase, PPCS
计算分子量 311 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC106064
基因名称 PPCS
Gene ID (NCBI) 79717
RRIDAB_10548725
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).

实验方案

Product Specific Protocols
WB protocol for PPCS antibody 18001-1-APDownload protocol
IHC protocol for PPCS antibody 18001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Regulation of the CoA Biosynthetic Complex Assembly in Mammalian Cells.

Authors - Jovana Baković
View Article