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MKRN3 Polyclonal antibody

MKRN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18349-1-AP

Print datasheet

Synonyms

D15S9, makorin ring finger protein 3, MKRN3, RING finger protein 63, RNF63, ZFP127, Zinc finger protein 127, ZNF127

验证数据展示

  • WB analysis using 18349-1-AP

    WB analysis using 18349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18349-1-AP (MKRN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 18349-1-AP

    IHC staining of mouse colon using 18349-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18349-1-AP (MKRN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 18349-1-AP

    IHC staining of mouse colon using 18349-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18349-1-AP (MKRN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 18349-1-AP

    IF Staining of A431 using 18349-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MKRN3 antibody (18349-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18349-1-AP targets MKRN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MKRN3 fusion protein Ag13167 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name makorin ring finger protein 3
Synonyms D15S9, makorin ring finger protein 3, MKRN3, RING finger protein 63, RNF63, ZFP127, Zinc finger protein 127, ZNF127
Calculated Molecular Weight 507 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC044639
Gene Symbol MKRN3
Gene ID (NCBI) 7681
RRIDAB_3669299
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13064
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MKRN3 antibody 18349-1-APDownload protocol
IHC protocol for MKRN3 antibody 18349-1-APDownload protocol
IF protocol for MKRN3 antibody 18349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols