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PLD1 Polyclonal antibody

PLD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18355-1-AP

Print datasheet

Synonyms

PLD 1, Phospholipase D1, Phosphatidylcholine-hydrolyzing phospholipase D1, hPLD1, EC:3.1.4.4

验证数据展示

  • WB analysis using 18355-1-AP

    WB analysis using 18355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18355-1-AP (PLD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 18355-1-AP

    IF Staining of HeLa using 18355-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLD1 antibody (18355-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse heart using 18355-1-AP

    IHC staining of mouse heart using 18355-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18355-1-AP (PLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLD1 antibody included

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PLD1 Ready-To-Use IHC Kit. PLD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18355-1-AP targets PLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PLD1 fusion protein Ag13227 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase D1, phosphatidylcholine-specific
Synonyms PLD 1, Phospholipase D1, Phosphatidylcholine-hydrolyzing phospholipase D1, hPLD1, EC:3.1.4.4
Calculated Molecular Weight 1074 aa, 124 kDa
Observed Molecular Weight112-124 kDa
GenBank Accession NumberBC068976
Gene Symbol PLD1
Gene ID (NCBI) 5337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13393
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.

实验方案

Product Specific Protocols
WB protocol for PLD1 antibody 18355-1-APDownload protocol
IHC protocol for PLD1 antibody 18355-1-APDownload protocol
IF protocol for PLD1 antibody 18355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Cell Biol

Class III PI-3-kinase activates phospholipase D in an amino acid-sensing mTORC1 pathway.

Authors - Yoon Mee-Sup MS
View Article