验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PLD1 Ready-To-Use IHC Kit. PLD1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
18355-1-AP targets PLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PLD1 fusion protein Ag13227 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phospholipase D1, phosphatidylcholine-specific |
| 别名 | PLD 1, Phospholipase D1, Phosphatidylcholine-hydrolyzing phospholipase D1, hPLD1, EC:3.1.4.4 |
| 计算分子量 | 1074 aa, 124 kDa |
| 观测分子量 | 112-124 kDa |
| GenBank蛋白编号 | BC068976 |
| 基因名称 | PLD1 |
| Gene ID (NCBI) | 5337 |
| RRID | AB_2163859 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13393 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLD1 antibody 18355-1-AP | Download protocol |
| IHC protocol for PLD1 antibody 18355-1-AP | Download protocol |
| IF protocol for PLD1 antibody 18355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |