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验证数据展示

  • IHC staining of human testis using 19180-1-AP

    IHC staining of human testis using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 19180-1-AP

    IHC staining of human testis using 19180-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis using 19180-1-AP

    WB analysis using 19180-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RUFY2 Polyclonal antibody

RUFY2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19180-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ZFYVE13, RUN and FYVE domain-containing protein 2, RABIP4R, Rab4-interacting protein related, KIAA1537

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, mouse lung tissue, mouse ovary tissue, SH-SY5Y cells, U-251 cells, rat lung tissue
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19180-1-AP targets RUFY2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RUFY2 fusion protein Ag5938 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RUN and FYVE domain containing 2
别名 ZFYVE13, RUN and FYVE domain-containing protein 2, RABIP4R, Rab4-interacting protein related, KIAA1537
计算分子量 403 aa, 46 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC041092
基因名称 RUFY2
Gene ID (NCBI) 55680
RRIDAB_10643382
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXA3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RUFY2 antibody 19180-1-APDownload protocol
IHC protocol for RUFY2 antibody 19180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols