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验证数据展示

  • IHC staining of human prostate cancer using 20104-1-AP

    IHC staining of human prostate cancer using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 20104-1-AP

    IHC staining of human prostate cancer using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human brain using 20104-1-AP

    WB analysis of human brain using 20104-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20104-1-AP

    IHC staining of human placenta using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20104-1-AP (HAUS4 antibody) at dilution of 1:100 (under 10x lens).

HAUS4 Polyclonal antibody

HAUS4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20104-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

C14orf94, HAUS4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, human placenta tissue, mouse lung tissue, mouse skin tissue
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20104-1-AP targets HAUS4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 HAUS4 fusion protein Ag13717 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 94
别名 C14orf94, HAUS4
计算分子量 363 aa, 42 kDa
观测分子量 28 kDa, 37 kDa, 42 kDa
GenBank蛋白编号BC002554
基因名称 C14orf94
Gene ID (NCBI) 54930
RRIDAB_10639366
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6D7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.

实验方案

Product Specific Protocols
WB protocol for HAUS4 antibody 20104-1-APDownload protocol
IHC protocol for HAUS4 antibody 20104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article