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验证数据展示

  • WB analysis of human plasma using 20311-1-AP

    WB analysis of human plasma using 20311-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 20311-1-AP (CLEC3B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 20311-1-AP

    IP experiment of human plasma using 20311-1-AP

    IP result of anti-CLEC3B (IP:20311-1-AP, 4ug; Detection:20311-1-AP 1:300) with human plasma lysate 4000ug.

  • IF Staining of HeLa using 20311-1-AP

    IF Staining of HeLa using 20311-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLEC3B antibody (20311-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tetranectin Polyclonal antibody

Tetranectin Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 20311-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

CLEC3B, TNA, TN, Plasminogen kringle 4-binding protein, C-type lectin domain family 3 member B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20311-1-AP targets Tetranectin in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 Tetranectin fusion protein Ag14147 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 C-type lectin domain family 3, member B
别名 CLEC3B, TNA, TN, Plasminogen kringle 4-binding protein, C-type lectin domain family 3 member B
计算分子量 202 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC011024
基因名称 CLEC3B
Gene ID (NCBI) 7123
RRIDAB_2878670
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-Type Lectin Domain Family 3 Member B (CLEC3B) is a transmembrane Ca2+-binding protein, locating in cell plasma, extracellular matrix and exosomes. Downregulation of CLEC3B has been reported in various diseases. It was demonstrated in CLEC3B-deficient mice that the absence of CLEC3B impeded the mineralization process in osteogenesis. (PMID: 31477130)

实验方案

Product Specific Protocols
WB protocol for Tetranectin antibody 20311-1-APDownload protocol
IF protocol for Tetranectin antibody 20311-1-APDownload protocol
IP protocol for Tetranectin antibody 20311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols