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验证数据展示

  • WB analysis using 20334-1-AP

    WB analysis using 20334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20334-1-AP (AQP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 20334-1-AP

    IHC staining of mouse lung using 20334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AQP5 antibody included
  • IHC staining of mouse lung using 20334-1-AP

    IHC staining of mouse lung using 20334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AQP5 antibody included
  • IF Staining of rat lung using 20334-1-AP

    IF Staining of rat lung using 20334-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AQP5 antibody (20334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AQP5 Polyclonal antibody

AQP5 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 20334-1-AP

产品说明书

引用文献(16)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

别名

aquaporin 5, Aquaporin-5, aquaporin5, AQP-5, AQP 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat lung tissue
Planning an IHC experiment? We recommend our IHCeasy AQP5 Ready-To-Use IHC Kit. AQP5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20334-1-AP targets AQP5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 AQP5 fusion protein Ag14103 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aquaporin 5
别名 aquaporin 5, Aquaporin-5, aquaporin5, AQP-5, AQP 5
计算分子量 265 aa, 28 kDa
观测分子量27 kDa
GenBank蛋白编号BC032946
基因名称 AQP5
Gene ID (NCBI) 362
RRIDAB_2918066
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55064
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AQP5 is a member of aquaporins (AQPs) which are membrane proteins functioning as water channels and involved in the bidirectional transfer of water and small solutes across cell membranes. AQP5 is widely expressed including digestive, renal, respiratory, and reproductive systems. AQP5 overexpression in cancer cells and tumor tissues has been extensively reported. Recently AQP5 has been identified as a marker for adult pyloric stem cells.

实验方案

Product Specific Protocols
WB protocol for AQP5 antibody 20334-1-APDownload protocol
IHC protocol for AQP5 antibody 20334-1-APDownload protocol
IF protocol for AQP5 antibody 20334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Ezrin Is a Novel Protein Partner of Aquaporin-5 in Human Salivary Glands and Shows Altered Expression and Cellular Localization in Sjögren's Syndrome

Authors - Clara Chivasso
View Article
mouseWB,IF

FASEB J

Circadian rhythm disruptions exacerbate inner ear damage in a murine endolymphatic hydrops model

Authors - Bo Pang
View Article
human,mouseWB,IHC

J Ethnopharmacol

Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway.

Authors - Xingbao Tao
View Article
IF

Front Genet

A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi).

Authors - Jimei Li
View Article
mouseIF

DNA Cell Biol

PANoptosis Features, a Humanized NSG Murine Model of Sjogren's Syndrome

Authors - Yiying Yang
View Article
humanWB

PLoS One

TRPML1 acts as a predisposing factor in lymphedema development by regulating the subcellular localization of aquaporin-3, -5

Authors - Lijie Yang
View Article