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验证数据展示

  • WB analysis of mouse testis using 20337-1-AP

    WB analysis of mouse testis using 20337-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20337-1-AP (TADA1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20337-1-AP

    IHC staining of human testis using 20337-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20337-1-AP (TADA1L Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 20337-1-AP

    IHC staining of human testis using 20337-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20337-1-AP (TADA1L Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20337-1-AP

    IF Staining of HepG2 using 20337-1-AP

    Immunofluorescent analysis of HepG2 cells using 20337-1-AP (TADA1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L).

TADA1L Polyclonal antibody

TADA1L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20337-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

TADA1, STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20337-1-AP targets TADA1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 TADA1L fusion protein Ag14118 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcriptional adaptor 1 (HFI1 homolog, yeast)-like
别名 TADA1, STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4
计算分子量 335 aa, 37 kDa
观测分子量 38 kDa
GenBank蛋白编号BC015401
基因名称 TADA1L
Gene ID (NCBI) 117143
RRIDAB_2878675
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96BN2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.The MW of this protein is 37 kDa, and Catalog#20337-1-AP specially recognises the 37 kDa protein.

实验方案

Product Specific Protocols
WB protocol for TADA1L antibody 20337-1-APDownload protocol
IHC protocol for TADA1L antibody 20337-1-APDownload protocol
IF protocol for TADA1L antibody 20337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genes Dev

The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

Authors - Ying-Jiun C Chen
View Article