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验证数据展示

  • WB analysis using 20562-1-AP

    WB analysis using 20562-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20562-1-AP (STXBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20562-1-AP

    IHC staining of human brain using 20562-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20562-1-AP (STXBP1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IHC staining of human brain using 20562-1-AP

    IHC staining of human brain using 20562-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20562-1-AP (STXBP1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IF Staining of HeLa using 20562-1-AP

    IF Staining of HeLa using 20562-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STXBP1 antibody (20562-1-AP) at dilution of 1:50 and CoraLite594-Conjugated Goat Anti-Rabbit IgG(H+L).

STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20562-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EIEE4, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20562-1-AP targets STXBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin binding protein 1
别名 EIEE4, MUNC18 1, N Sec1, p67, Protein unc 18 homolog 1, Protein unc 18 homolog A, RBSEC1, STXBP1, syntaxin binding protein 1, Unc 18A, UNC18, Unc18 1, UNC18A
计算分子量 68 kDa
观测分子量68 kDa
GenBank蛋白编号NM_003165
基因名称 STXBP1
Gene ID (NCBI) 6812
RRIDAB_10694824
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61764
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions. The antibody is specific to STXBP1.

实验方案

Product Specific Protocols
WB protocol for STXBP1 antibody 20562-1-APDownload protocol
IHC protocol for STXBP1 antibody 20562-1-APDownload protocol
IF protocol for STXBP1 antibody 20562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols