- Featured Product
- KD/KO Validated
PTPIP51 Polyclonal antibody
PTPIP51 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human paracancerous of skin cancer, human cervical cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20641-1-AP targets PTPIP51 in WB, IHC, IF, IP, COIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, COIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, monkey |
| Immunogen | PTPIP51 fusion protein Ag14708 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | family with sequence similarity 82, member A2 |
| Synonyms | FAM82A2, Protein FAM82A2, hucep-10, hRMD-3, hRMD 3 |
| Calculated Molecular Weight | 470 aa, 52 kDa |
| Observed Molecular Weight | 52-60 kDa |
| GenBank Accession Number | BC008970 |
| Gene Symbol | PTPIP51 |
| Gene ID (NCBI) | 55177 |
| RRID | AB_10695187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96TC7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618). The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
| IHC protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
| IP protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
| ||
Mol Cell The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. | ||
J Cell Biol Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. | ||
EMBO Rep Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling
| ||
J Cell Sci The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo. |
