验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, U2OS cells, C2C12 cells |
| Positive IHC detected in | human liver cancer tissue, human normal colon, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20685-1-AP targets Filamin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | filamin B, beta (actin binding protein 278) |
| 别名 | FLNB, FilaminB, Filamin-3, Beta-filamin, Actin-binding-like protein |
| 计算分子量 | 278 kDa |
| 观测分子量 | 250-278 kDa |
| GenBank蛋白编号 | NM_001457 |
| 基因名称 | FLNB |
| Gene ID (NCBI) | 2317 |
| RRID | AB_10863658 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75369 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. The antibody is specific to FLNB. It can recognize isoform1,2,3,6,7 of FLNB. It has no cross reaction to FLNA and FLNC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Filamin B antibody 20685-1-AP | Download protocol |
| IHC protocol for Filamin B antibody 20685-1-AP | Download protocol |
| IF protocol for Filamin B antibody 20685-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90. | ||
Heliyon Filamin B knockdown impairs differentiation and function in mouse pre-osteoblasts via aberrant transcription and alternative splicing
| ||
J Genet Genomics Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts | ||
Am J Cancer Res FLNB overexpression promotes tumor progression and associates with immune suppression, evasion and stemness in pancreatic cancer |
