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验证数据展示

  • IHC staining of human lymphoma using 21028-1-AP

    IHC staining of human lymphoma using 21028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with RNF213 antibody included
  • IHC staining of human lymphoma using 21028-1-AP

    IHC staining of human lymphoma using 21028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with RNF213 antibody included
  • WB analysis using 21028-1-AP

    WB analysis using 21028-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21028-1-AP (RNF213 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HUVEC using 21028-1-AP

    IP experiment of HUVEC using 21028-1-AP

    IP result of anti-RNF213 (IP:21028-1-AP, 4ug; Detection:21028-1-AP 1:300) with HUVEC cells lysate 910 ug.

RNF213 Polyclonal antibody

RNF213 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 21028-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

ring finger protein 213, Protein ALO17, KIAA1618, KIAA1554, C17orf27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells
Positive IP detected inHUVEC cells
Positive IHC detected inhuman lymphoma tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RNF213 Ready-To-Use IHC Kit. RNF213 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

21028-1-AP targets RNF213 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原 RNF213 fusion protein Ag15335 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 213
别名 ring finger protein 213, Protein ALO17, KIAA1618, KIAA1554, C17orf27
计算分子量 3280 aa, 374 kDa
GenBank蛋白编号BC032220
基因名称 RNF213
Gene ID (NCBI) 57674
RRIDAB_2878793
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ63HN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF213 is a typical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PMID: 21799892, 26126547, 26278786, 26766444, 30705059).RNF213 contains a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions.

实验方案

Product Specific Protocols
WB protocol for RNF213 antibody 21028-1-APDownload protocol
IHC protocol for RNF213 antibody 21028-1-APDownload protocol
IP protocol for RNF213 antibody 21028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Thorac Cancer

RNF213 gene mutation in circulating tumor DNA detected by targeted next-generation sequencing in the assisted discrimination of early-stage lung cancer from pulmonary nodules.

Authors - Ning Jiang
View Article