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验证数据展示

  • WB analysis of pig cartilage using 21136-1-AP

    WB analysis of pig cartilage using 21136-1-AP

    pig cartilage tissue was subjected to SDS PAGE followed by western blot with 21136-1-AP (Fibulin-7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Fibulin-7 Polyclonal antibody

Fibulin-7 Polyclonal Antibody for WB, ELISA
Cat No. 21136-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, ELISA

别名

FBLN7, FIBL 7, fibulin 7, TM14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig cartilage tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21136-1-AP targets Fibulin-7 in WB, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, pig
免疫原 Fibulin-7 fusion protein Ag15564 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibulin 7
别名 FBLN7, FIBL 7, fibulin 7, TM14
计算分子量 439 aa, 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC035784
基因名称 Fibulin-7
Gene ID (NCBI) 129804
RRIDAB_3085638
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ53RD9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibulins are a family of extracellular matrix glycoproteins associated with basement membranes, elastic fibers, and other matrices (PMID: 19189051). The family members are defined by the presence of two structural modules, a tandem repeat of epidermal growth factor-like modules, and a unique C-terminal fibulin-type module (PMID: 19189051). They play an important role in regulating multiple cellular functions such as adhesion, growth, motility, and survival (PMID: 32429873). Fibulin-7, also named as TM14, was first identified in the mouse tooth germ cDNA microarray by differential hybridization (PMID: 17699513). Fibulin-7 is expressed in developing odontoblasts, in the giant trophoblast layer of the placenta, in the choroid of the eyes as well as in the cartilage (PMID: 32429873). It plays important roles in both the differentiation and maintenance of odontoblasts as well as in dentin formation.

实验方案

Product Specific Protocols
WB protocol for Fibulin-7 antibody 21136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols