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验证数据展示

  • IHC staining of human colon using 21370-1-AP

    IHC staining of human colon using 21370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 21370-1-AP

    IHC staining of human colon using 21370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of MCF-7 using 21370-1-AP

    WB analysis of MCF-7 using 21370-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PLCB3 Polyclonal antibody

PLCB3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21370-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Phospholipase C beta 3, PLC beta 3, PLCB3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, A431 cells, COLO 320 cells, HEK-293T cells, NIH/3T3 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21370-1-AP targets PLCB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PLCB3 fusion protein Ag15845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, beta 3 (phosphatidylinositol-specific)
别名 Phospholipase C beta 3, PLC beta 3, PLCB3
计算分子量 1234 aa, 139 kDa
观测分子量 150 kDa
GenBank蛋白编号BC142681
基因名称 PLCB3
Gene ID (NCBI) 5331
RRIDAB_2878848
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01970
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCB3 antibody 21370-1-APDownload protocol
IHC protocol for PLCB3 antibody 21370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols