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验证数据展示

  • WB analysis of HeLa using 21392-1-AP

    WB analysis of HeLa using 21392-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21392-1-AP (NCKAP5L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 21392-1-AP

    IHC staining of human skin using 21392-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21392-1-AP (NCKAP5L antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 21392-1-AP

    IHC staining of human skin using 21392-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21392-1-AP (NCKAP5L antibody) at dilution of 1:50 (under 40x lens).

NCKAP5L Polyclonal antibody

NCKAP5L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21392-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Cep169, FP1193, KIAA1602, Nck associated protein 5 like, NCKAP5L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21392-1-AP targets NCKAP5L in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 NCKAP5L fusion protein Ag16050 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1602
别名 Cep169, FP1193, KIAA1602, Nck associated protein 5 like, NCKAP5L
计算分子量 1334 aa, 139 kDa
观测分子量 145-150 kDa
GenBank蛋白编号BC110599
基因名称 NCKAP5L
Gene ID (NCBI) 57701
RRIDAB_10860259
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCH0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCKAP5L, also named as KIAA1602, has 4 isoforms. The target band is about 139-145 kDa for phosphorylation.

实验方案

Product Specific Protocols
WB protocol for NCKAP5L antibody 21392-1-APDownload protocol
IHC protocol for NCKAP5L antibody 21392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols