验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
21436-1-AP targets CLSTN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | CLSTN2 fusion protein Ag16048 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calsyntenin 2 |
| 别名 | Calsyntenin-2, calsyntenin 2, Alc-gamma, Alcadein-gamma, Alcadein gamma |
| 计算分子量 | 955 aa, 107 kDa |
| 观测分子量 | 100-107 kDa |
| GenBank蛋白编号 | BC104485 |
| 基因名称 | CLSTN2 |
| Gene ID (NCBI) | 64084 |
| RRID | AB_3085656 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H4D0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CLSTN2 gene encodes the synaptic protein calsyntenin 2, one of the three calsyntenin family members found in humans. CLSTN2 is expressed exclusively in the brain, with high levels occurring in cortical gamma-aminobutyric acid (GABA) interneurons and in medial temporal lobe regions (PMID: 19058786). Western blot analysis detected CLSTN2 at an apparent molecular mass of 107 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLSTN2 antibody 21436-1-AP | Download protocol |
| IHC protocol for CLSTN2 antibody 21436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |