验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21609-1-AP targets IL-1-RAPL-1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | IL-1-RAPL-1 fusion protein Ag16252 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 1 receptor accessory protein-like 1 |
| 别名 | IL1RAPL1, IL1RAPL 1, IL1RAPL, IL1R8, IL 1RAPL 1 |
| 计算分子量 | 696 aa, 80 kDa |
| 观测分子量 | 80-100 kDa |
| GenBank蛋白编号 | BC126345 |
| 基因名称 | IL-1-RAPL-1 |
| Gene ID (NCBI) | 11141 |
| RRID | AB_10888632 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZN1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-1-RAPL-1 antibody 21609-1-AP | Download protocol |
| IHC protocol for IL-1-RAPL-1 antibody 21609-1-AP | Download protocol |
| IF protocol for IL-1-RAPL-1 antibody 21609-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.
| ||
Allergy Skewed inflammation is associated with aberrant interleukin-37 signaling pathway in atopic dermatitis. | ||
EMBO J Splice-dependent trans-synaptic PTPδ-IL1RAPL1 interaction regulates synapse formation and non-REM sleep. | ||
Cell Death Dis IL-37 isoform D downregulates pro-inflammatory cytokines expression in a Smad3-dependent manner.
| ||
J Neurosci The X-linked intellectual disability protein IL1RAPL1 regulates dendrite complexity.
| ||
Cell Rep IL-38 Ameliorates Skin Inflammation and Limits IL-17 Production from γδ T Cells. |
