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验证数据展示

  • WB analysis of HepG2 using 21778-1-AP

    WB analysis of HepG2 using 21778-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21778-1-AP

    IF Staining of HeLa using 21778-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21778-1-AP (POLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

POLA2 Polyclonal antibody

POLA2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21778-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA and More (1)

别名

DNA polymerase alpha subunit B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21778-1-AP targets POLA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 POLA2 fusion protein Ag0271 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), alpha 2 (70kD subunit)
别名 DNA polymerase alpha subunit B
计算分子量 70 kDa
观测分子量 68-70 kDa
GenBank蛋白编号BC001347
基因名称 POLA2
Gene ID (NCBI) 23649
RRIDAB_10733243
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14181
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLA2 antibody 21778-1-APDownload protocol
IF protocol for POLA2 antibody 21778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

CST does not evict elongating telomerase but prevents initiation by ssDNA binding.

Authors - Arthur J Zaug
View Article
humanIHC

Cancer Cell Int

Overexpression of POLA2 in hepatocellular carcinoma is involved in immune infiltration and predicts a poor prognosis

Authors - Long Liu
View Article
humanWB

Transl Cancer Res

E2F-mediated POLA2 upregulation is correlated with macrophage infiltration and poor prognosis in hepatocellular carcinoma

Authors - Yao Teng
View Article