Vimentin Polyclonal antibody
Vimentin Polyclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, mouse heart tissue |
Positive IHC detected in | human ovary tumor tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human kidney tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22031-1-AP targets Vimentin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF-P, IHC, WB, ELISA |
Tested Reactivity | human, mouse, rat |
Immunogen | Vimentin fusion protein Ag16898 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | vimentin |
Synonyms | Cell and organelle markers, Cytoskeleton Marker, VIM, vimentin |
Calculated Molecular Weight | 466 aa, 54 kDa |
Observed Molecular Weight | 57 kDa |
GenBank Accession Number | BC000163 |
Gene Symbol | VIM |
Gene ID (NCBI) | 7431 |
ENSEMBL Gene ID | ENSG00000026025 |
RRID | AB_11182825 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08670 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Vimentin antibody 22031-1-AP | Download protocol |
IHC protocol for Vimentin antibody 22031-1-AP | Download protocol |
IF protocol for Vimentin antibody 22031-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |