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验证数据展示

  • WB analysis using 22048-1-AP

    WB analysis using 22048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse placenta using 22048-1-AP

    WB analysis of mouse placenta using 22048-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 22048-1-AP

    IHC staining of human heart using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 22048-1-AP

    IHC staining of human heart using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 40x lens).

B3GALNT1 Polyclonal antibody

B3GALNT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22048-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

b3Gal T3, B3GALNT1, B3GALT3, Beta 1,3 GalNAc T1, Beta 1,3 GalTase 3, Beta 3 Gx T3, beta3Gal T3, Beta3GalT3, galT3, Gb4Cer, GLCT3, GLOB, Globoside synthase, P1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse placenta tissue, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22048-1-AP targets B3GALNT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 B3GALNT1 fusion protein Ag16976 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
别名 b3Gal T3, B3GALNT1, B3GALT3, Beta 1,3 GalNAc T1, Beta 1,3 GalTase 3, Beta 3 Gx T3, beta3Gal T3, Beta3GalT3, galT3, Gb4Cer, GLCT3, GLOB, Globoside synthase, P1
计算分子量 40 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC047618
基因名称 B3GALNT1
Gene ID (NCBI) 8706
RRIDAB_11182184
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75752
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for B3GALNT1 antibody 22048-1-APDownload protocol
IHC protocol for B3GALNT1 antibody 22048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols