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验证数据展示

  • WB analysis using 22352-1-AP

    WB analysis using 22352-1-AP

    Untreated, PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 22352-1-AP (CCL4/MIP-1 beta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CCL4/MIP-1 beta Polyclonal antibody

CCL4/MIP-1 beta Polyclonal Antibody for WB, ELISA
Cat No. 22352-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ACT 2, ACT2, AT744.1, C C motif chemokine 4, CCL4, chemokine (C C motif) ligand 4, G 26, HC21, LAG 1, LAG1, MIP 1 beta, MIP 1 beta(1 69), MIP-1 Beta, MIP1B, MIP1B1, PAT 744, Protein H400, SCYA2, SCYA4, SIS gamma, Small inducible cytokine A4, T cell activation protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22352-1-AP targets CCL4/MIP-1 beta in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CCL4/MIP-1 beta fusion protein Ag17928 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 4
别名 ACT 2, ACT2, AT744.1, C C motif chemokine 4, CCL4, chemokine (C C motif) ligand 4, G 26, HC21, LAG 1, LAG1, MIP 1 beta, MIP 1 beta(1 69), MIP-1 Beta, MIP1B, MIP1B1, PAT 744, Protein H400, SCYA2, SCYA4, SIS gamma, Small inducible cytokine A4, T cell activation protein 2
计算分子量 92 aa, 10 kDa
观测分子量11 kDa
GenBank蛋白编号BC107433
基因名称 CCL4/MIP-1 beta
Gene ID (NCBI) 6351
RRIDAB_3669396
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13236
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β), is a CC chemokine with specificity for CCR5 receptors, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. MIP-1β is an acidic protein composed of 69 amino acids that is produced by many cells, particularly macrophages, dendritic cells, and lymphocytes. MIP-1β is responsible for the activation of PMN and is involved in acute neutrophilic inflammation. Recombinant MIP-1β induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

实验方案

Product Specific Protocols
WB protocol for CCL4/MIP-1 beta antibody 22352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols