验证数据展示
经过测试的应用
Positive IHC detected in | human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22578-1-AP targets ARAP3 in IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | ARAP3 fusion protein Ag17436 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 |
别名 | ARAP3, Centaurin delta 3, CENTD3, Cnt d3, DRAG1 |
计算分子量 | 1544 aa, 170 kDa |
GenBank蛋白编号 | BC009968 |
基因名称 | ARAP3 |
Gene ID (NCBI) | 64411 |
RRID | AB_2879125 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8WWN8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. These proteins are best characterized as regulators of membrane traffic. The Centaurin GTPase-activating protein family comprise a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of ARF-dependent pathways. ARAP3 (Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3) is unique for its dual specificity GAPs (GTPase activating protein) activity for Arf6 (ADP-ribosylation factor 6) and RhoA (Ras homolog gene family member A) regulated by PtdIns(3,4,5)P3 (phosphatidylinositol (3,4,5)-trisphosphate) and a small GTPase Rap1-GTP and is involved in regulation of cell shape and adhesion.
实验方案
Product Specific Protocols | |
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IHC protocol for ARAP3 antibody 22578-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |