验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
22722-1-AP targets WHSC1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | WHSC1 fusion protein Ag18611 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Wolf-Hirschhorn syndrome candidate 1 |
| 别名 | KIAA1090, MMSET, NSD2, Protein trithorax 5, REIIBP, TRX5, WHS, WHSC1 |
| 计算分子量 | 162 kDa |
| 观测分子量 | 70 kDa, 150 kDa |
| GenBank蛋白编号 | BC152412 |
| 基因名称 | WHSC1 |
| Gene ID (NCBI) | 7468 |
| RRID | AB_2879156 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | O96028 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WHSC1 (also known as MMSET or NSD2) is a SET domain containing histone lysine methyltransferase that can di- and trimethylate histone H3 at lysine 36 (H3K36). WHSC1 mRNA is highly expressed in embryonic tissues, especially those with high degrees of proliferation, whereas in adult tissues,the mRNA is primarily expressed in thymus and testis. WHSC1 is implicated in cellular adhesion, clonogenic growth and tumorigenicity. Its overexpression has been found in various cancers. It undergoes alternative splicing resulting in different protein isoforms. Recently WHSC1 has been identified as a critical epigenetic regulator for Tfh differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WHSC1 antibody 22722-1-AP | Download protocol |
| IHC protocol for WHSC1 antibody 22722-1-AP | Download protocol |
| IP protocol for WHSC1 antibody 22722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |