验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22815-1-AP targets SLCO1B3/OATP1B3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SLCO1B3/OATP1B3 fusion protein Ag18940 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier organic anion transporter family, member 1B3 |
| 别名 | LST 2, LST 3TM13, LST2, LST3, OATP 8, OATP1B3, OATP8, Organic anion transporter 8, SLC21A8, SLCO1B3 |
| 计算分子量 | 702 aa, 77 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC105597 |
| 基因名称 | SLCO1B3 |
| Gene ID (NCBI) | 28234 |
| RRID | AB_3669405 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NPD5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLCO1B3, also known as OATP8 or OATP1B3, is a member of Organic Anion Transporting Polypeptides (OATPs) which are sodium-independent organic anion transporters mediating the uptake of a wide range of structurally diverse endogenous and exogenous compounds including bile acids, hormone conjugates, peptides, toxins, as well as a multitude of therapeutic drugs. SLCO1B3 is specifically expressed in liver under normal condition, while its expression has also been observed in cancer tissues like colon, prostate and pancreas. SLCO1B3 is a glycosylated protein and can be detected in the non-glycosylated form at 70-75 kDa(PMID: 21278621).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLCO1B3/OATP1B3 antibody 22815-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |