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ESRP2 Polyclonal antibody

ESRP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23117-1-AP

Print datasheet

Synonyms

ESRP2, RBM35B, RNA binding motif protein 35B, RNA binding protein 35B

验证数据展示

  • WB analysis using 23117-1-AP

    WB analysis using 23117-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23117-1-AP (ESRP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 23117-1-AP

    IHC staining of human breast cancer using 23117-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23117-1-AP (ESRP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23117-1-AP targets ESRP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ESRP2 fusion protein Ag19436 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epithelial splicing regulatory protein 2
Synonyms ESRP2, RBM35B, RNA binding motif protein 35B, RNA binding protein 35B
Calculated Molecular Weight 727 aa, 78 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC030146
Gene Symbol ESRP2
Gene ID (NCBI) 80004
RRIDAB_2879213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6T0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ESRP2 (Epithelial Splicing Regulatory Protein 2), also known as RBM35B(RNA binding motif protein 35B), is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family of RNA binding proteins. ESRP2 plays a role in the regulation of alternative splicing events of pre-mRNAs(PMID: 27401076). Overexpression of ESRP2 has been described in various malignant tumors, such as pancreatic ductal adenocarcinoma, oral squamous carcinoma, ovarian cancer, and luminal-type breast cancer(PMID: 33339518).

实验方案

Product Specific Protocols
WB protocol for ESRP2 antibody 23117-1-APDownload protocol
IHC protocol for ESRP2 antibody 23117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Oncogene

Androgen receptor signaling regulates the transcriptome of prostate cancer cells by modulating global alternative splicing.

Authors - Kalpit Shah
View Article