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验证数据展示

  • WB analysis using 23174-1-AP

    WB analysis using 23174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23174-1-AP (ZFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 23174-1-AP

    IHC staining of human heart using 23174-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23174-1-AP (ZFR Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 23174-1-AP

    IHC staining of human small intestine using 23174-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23174-1-AP (ZFR Antibody) at dilution of 1:50 (under 10x lens).

ZFR Polyclonal antibody

ZFR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23174-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Zinc finger RNA-binding protein, ZFR1, M-phase phosphoprotein homolog, M phase phosphoprotein homolog, hZFR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman small intestine tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23174-1-AP targets ZFR in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ZFR fusion protein Ag19559 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger RNA binding protein
别名 Zinc finger RNA-binding protein, ZFR1, M-phase phosphoprotein homolog, M phase phosphoprotein homolog, hZFR
计算分子量 1074 aa, 117 kDa
观测分子量120 kDa
GenBank蛋白编号BC137085
基因名称 ZFR
Gene ID (NCBI) 51663
RRIDAB_3085708
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96KR1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZFR antibody 23174-1-APDownload protocol
IHC protocol for ZFR antibody 23174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols