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ZFR Polyclonal antibody

ZFR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23174-1-AP

Print datasheet

Synonyms

Zinc finger RNA-binding protein, ZFR1, M-phase phosphoprotein homolog, M phase phosphoprotein homolog, hZFR

验证数据展示

  • WB analysis using 23174-1-AP

    WB analysis using 23174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23174-1-AP (ZFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 23174-1-AP

    IHC staining of human heart using 23174-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23174-1-AP (ZFR Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 23174-1-AP

    IHC staining of human small intestine using 23174-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23174-1-AP (ZFR Antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman small intestine tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23174-1-AP targets ZFR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ZFR fusion protein Ag19559 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger RNA binding protein
Synonyms Zinc finger RNA-binding protein, ZFR1, M-phase phosphoprotein homolog, M phase phosphoprotein homolog, hZFR
Calculated Molecular Weight 1074 aa, 117 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC137085
Gene Symbol ZFR
Gene ID (NCBI) 51663
RRIDAB_3085708
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96KR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZFR antibody 23174-1-APDownload protocol
IHC protocol for ZFR antibody 23174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols