验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, L02 cells, Neuro-2a cells, NIH/3T3 cells, rat liver tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23217-1-AP targets LRRC47 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | LRRC47 fusion protein Ag19736 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine rich repeat containing 47 |
| 别名 | Leucine-rich repeat-containing protein 47, KIAA1185 |
| 计算分子量 | 583 aa, 63 kDa |
| 观测分子量 | 63 kDa |
| GenBank蛋白编号 | BC031301 |
| 基因名称 | LRRC47 |
| Gene ID (NCBI) | 57470 |
| RRID | AB_2879234 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q8N1G4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LRRC47 antibody 23217-1-AP | Download protocol |
| IHC protocol for LRRC47 antibody 23217-1-AP | Download protocol |
| IF protocol for LRRC47 antibody 23217-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Structural basis for the final steps of human 40S ribosome maturation.
| ||
bioRxiv LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins
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