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验证数据展示

  • IP experiment of human testis using 23314-1-AP

    IP experiment of human testis using 23314-1-AP

    IP result of anti-STAG3 (IP:23314-1-AP, 4ug; Detection:23314-1-AP 1:300) with human testis tissue lysate 4000ug.

  • IHC staining of human testis using 23314-1-AP

    IHC staining of human testis using 23314-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of A549 using 23314-1-AP

    IF Staining of A549 using 23314-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 23314-1-AP (STAG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

STAG3 Polyclonal antibody

STAG3 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 23314-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IP, ELISA and More (1)

别名

Cohesin subunit SA 3, SCC3 homolog 3, STAG3, stromal antigen 3, Stromalin 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23314-1-AP targets STAG3 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
文献引用应用IF, IHC
经测试反应性 human
文献引用反应性human
免疫原 STAG3 fusion protein Ag19818 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stromal antigen 3
别名 Cohesin subunit SA 3, SCC3 homolog 3, STAG3, stromal antigen 3, Stromalin 3
计算分子量 1225 aa, 139 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC146806
基因名称 STAG3
Gene ID (NCBI) 10734
RRIDAB_2879253
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJ98
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for STAG3 antibody 23314-1-APDownload protocol
IF protocol for STAG3 antibody 23314-1-APDownload protocol
IP protocol for STAG3 antibody 23314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL).

Authors - Jennifer Gantchev
View Article
humanIF

Front Genet

In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency.

Authors - Wen-Juan Xiao
View Article