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验证数据展示

  • IF Staining of mouse eye using 23566-1-AP

    IF Staining of mouse eye using 23566-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using 23566-1-AP (VSX1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse eye using 23566-1-AP

    WB analysis of mouse eye using 23566-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 23566-1-AP (VSX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

VSX1 Polyclonal antibody

VSX1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 23566-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

Homeodomain protein RINX, KTCN, PPCD, PPD, RINX, Transcription factor VSX1, visual system homeobox 1, VSX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue, Raji cells
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23566-1-AP targets VSX1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 VSX1 fusion protein Ag19222 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 visual system homeobox 1
别名 Homeodomain protein RINX, KTCN, PPCD, PPD, RINX, Transcription factor VSX1, visual system homeobox 1, VSX1
计算分子量 365 aa, 38 kDa
观测分子量 70 kDa
GenBank蛋白编号BC126228
基因名称 VSX1
Gene ID (NCBI) 30813
RRIDAB_2879297
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZR4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VSX1(visual system homeobox 1) is a member of the Vsx1 group of vertebrate paired-like homeodomain transcription factors locating to the nucleus. Mutations in the VSX1 gene in keratoconus have been reported in many studies. VSX1 is considered important in ocular development and is particularly involved in the developing cornea (PMID:21139977). And previous studies have identified that Vsx1 participates in regulating retinal progenitor proliferation, differentiation and functional maintenance of bipolar cells (PMID:25150888).It was demonstrated that Vsx1 express in embryonic craniofacial, adult corneal, and adult retinal cDNA libraries.VSX1 mRNA has been found in the outer tier of the inner nuclear layer of the human retina and the cornea (PMID: 21139977).

实验方案

Product Specific Protocols
WB protocol for VSX1 antibody 23566-1-APDownload protocol
IF protocol for VSX1 antibody 23566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols