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验证数据展示

  • WB analysis using 23569-1-AP

    WB analysis using 23569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23569-1-AP (PEG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 23569-1-AP

    IHC staining of human placenta using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 23569-1-AP

    IHC staining of mouse brain using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 23569-1-AP

    IHC staining of human placenta using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PEG3 Polyclonal antibody

PEG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23569-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

KIAA0287, paternally expressed 3, PEG3, PW1, ZSCAN24

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, TT cells
Positive IHC detected inhuman placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23569-1-AP targets PEG3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PEG3 fusion protein Ag20132 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 paternally expressed 3
别名 KIAA0287, paternally expressed 3, PEG3, PW1, ZSCAN24
计算分子量 1588 aa, 181 kDa
观测分子量200-220 kDa
GenBank蛋白编号BC150272
基因名称 PEG3
Gene ID (NCBI) 5178
RRIDAB_3085712
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9GZU2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).

实验方案

Product Specific Protocols
WB protocol for PEG3 antibody 23569-1-APDownload protocol
IHC protocol for PEG3 antibody 23569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols