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PEG3 Polyclonal antibody

PEG3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23569-1-AP

Print datasheet

Synonyms

KIAA0287, paternally expressed 3, PEG3, PW1, ZSCAN24

验证数据展示

  • WB analysis using 23569-1-AP

    WB analysis using 23569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23569-1-AP (PEG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 23569-1-AP

    IHC staining of human placenta using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 23569-1-AP

    IHC staining of mouse brain using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 23569-1-AP

    IHC staining of human placenta using 23569-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, TT cells
Positive IHC detected inhuman placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23569-1-AP targets PEG3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PEG3 fusion protein Ag20132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name paternally expressed 3
Synonyms KIAA0287, paternally expressed 3, PEG3, PW1, ZSCAN24
Calculated Molecular Weight 1588 aa, 181 kDa
Observed Molecular Weight200-220 kDa
GenBank Accession NumberBC150272
Gene Symbol PEG3
Gene ID (NCBI) 5178
RRIDAB_3085712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9GZU2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).

实验方案

Product Specific Protocols
WB protocol for PEG3 antibody 23569-1-APDownload protocol
IHC protocol for PEG3 antibody 23569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols