验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23892-1-AP targets N4BP2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | N4BP2 fusion protein Ag20943 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NEDD4 binding protein 2 |
| 别名 | NEDD4 binding protein 2, KIAA1413, B3BP |
| 计算分子量 | 1770 aa, 199 kDa |
| 观测分子量 | 199 kDa |
| GenBank蛋白编号 | BC126466 |
| 基因名称 | N4BP2 |
| Gene ID (NCBI) | 55728 |
| RRID | AB_3669422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q86UW6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
N4BP2 (Nedd4 binding protein 2) is a Bcl-3 binding protein, which contains a polynucleotide kinase domain (PNK) at the N-terminus and a Small MutS Related (Smr) domain with nicking endonuclease activity near C-terminus. N4BP2 was at higher levels in a majority of tumor tissues examined, relative to paired normal tissues (PMID: 17626640).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for N4BP2 antibody 23892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |