验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue |
| Positive IP detected in | human plasma tissue |
| Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 3 publications below |
产品信息
24295-1-AP targets Coagulation Factor II in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, plasmodium falciparum |
| 免疫原 | Coagulation Factor II fusion protein Ag18385 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coagulation factor II (prothrombin) |
| 别名 | Prothrombin, F2, PT, EC:3.4.21.5, Activation peptide fragment 2 |
| 计算分子量 | 622 aa, 70 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC051332 |
| 基因名称 | F2 |
| Gene ID (NCBI) | 2147 |
| RRID | AB_2879478 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00734 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Coagulation Factor II antibody 24295-1-AP | Download protocol |
| IF protocol for Coagulation Factor II antibody 24295-1-AP | Download protocol |
| IP protocol for Coagulation Factor II antibody 24295-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Sci Rep Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. | ||
Sci Rep C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1. | ||
Malar J The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. | ||
PLoS Pathog Thrombin cleavage of the hepatitis E virus polyprotein at multiple conserved locations is required for genome replication |