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验证数据展示

  • WB analysis of rat retina using 24430-1-AP

    WB analysis of rat retina using 24430-1-AP

    rat retina tissue were subjected to SDS PAGE followed by western blot with 24430-1-AP (RS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse eye using 24430-1-AP

    WB analysis of mouse eye using 24430-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 24430-1-AP (RS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 24430-1-AP

    IHC staining of mouse eye using 24430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 24430-1-AP

    IHC staining of mouse eye using 24430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 24430-1-AP

    IF Staining of mouse eye using 24430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RS1 antibody (24430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 24430-1-AP

    IF Staining of mouse eye using 24430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RS1 antibody (24430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 24430-1-AP

    IF Staining of mouse eye using 24430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse eye tissue using 24430-1-AP (RS1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RS1 Polyclonal antibody

RS1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 24430-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, ELISA and More (1)

别名

XLRS1, Retinoschisin, retinoschisin 1, X linked juvenile retinoschisis protein, X-linked juvenile retinoschisis protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat retina tissue, mouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Positive IF-Fro detected inmouse eye tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24430-1-AP targets RS1 in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 RS1 fusion protein Ag18885 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinoschisin 1
别名 XLRS1, Retinoschisin, retinoschisin 1, X linked juvenile retinoschisis protein, X-linked juvenile retinoschisis protein
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC141638
基因名称 RS1
Gene ID (NCBI) 6247
RRIDAB_2879545
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15537
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RS1 is also named as XLRS1. RS1 can bind negatively charged membrane lipids, such as phosphatidylserine and phosphoinositides. RS1 is required for normal structure and function of the retina (PMID:19093009). It may play a role in cell-cell adhesion processes in the retina (PMID:27114531). RS1 is high expression in the retina (at protein level) (PMID:10915776, PMID:9326935). It is detected in the inner segment of the photoreceptors, the inner nuclear layer, the inner plexiform layer and the ganglion cell layer. At the macula, it is expressed in both the outer and inner nuclear layers and in the inner plexiform layer (PMID:10915776, PMID:10915776). And RS1 is undetectable in the inner plexiform layers and the inner nuclear layer (PMID:10915776)

实验方案

Product Specific Protocols
WB protocol for RS1 antibody 24430-1-APDownload protocol
IHC protocol for RS1 antibody 24430-1-APDownload protocol
IF protocol for RS1 antibody 24430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Biochem Biophys Rep

Intravitreal injection of new adeno-associated viral vector: Enhancing retinoschisin 1 gene transduction in a mouse model of X-linked retinoschisis

Authors - Yan Sun
View Article
mouseWB

Hum Gene Ther

Intravitreal Delivery of rAAV2-hSyn-hRS1 Results in Retinal Ganglion Cell-Specific Gene Expression and Retinal Improvement in the Rs1-KO Mouse

Authors - Yangyang Zheng
View Article