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验证数据展示

  • WB analysis using 24440-1-AP

    WB analysis using 24440-1-AP

    Mouse Heart tissue lysates were subjected to SDS PAGE followed by western blot with 24440-1-AP (IRX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24440-1-AP

    IHC staining of human colon cancer using 24440-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24440-1-AP (IRX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24440-1-AP

    IHC staining of human colon cancer using 24440-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24440-1-AP (IRX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IRX6 Polyclonal antibody

IRX6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24440-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

IRXB3, IRX7, IRX 3, Iroquois-class homeodomain protein IRX-6, iroquois homeobox 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24440-1-AP targets IRX6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 IRX6 fusion protein Ag19623 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 iroquois homeobox 6
别名 IRXB3, IRX7, IRX 3, Iroquois-class homeodomain protein IRX-6, iroquois homeobox 6
计算分子量 446 aa, 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC136573
基因名称 IRX6
Gene ID (NCBI) 79190
RRIDAB_2918082
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78412
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRX6 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.

实验方案

Product Specific Protocols
WB protocol for IRX6 antibody 24440-1-APDownload protocol
IHC protocol for IRX6 antibody 24440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols