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验证数据展示

  • WB analysis using 24691-1-AP

    WB analysis using 24691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24691-1-AP (EYA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 24691-1-AP

    IHC staining of human skeletal muscle using 24691-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24691-1-AP (EYA4 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with EYA4 antibody included
  • IHC staining of human skeletal muscle using 24691-1-AP

    IHC staining of human skeletal muscle using 24691-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24691-1-AP (EYA4 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with EYA4 antibody included
  • IF Staining of HeLa using 24691-1-AP

    IF Staining of HeLa using 24691-1-AP

    Immunofluorescent analysis of HeLa cells using 24691-1-AP (EYA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EYA4 Polyclonal antibody

EYA4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24691-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

CMD1J, DFNA10, EYA4, Eyes absent homolog 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EYA4 Ready-To-Use IHC Kit. EYA4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24691-1-AP targets EYA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, pig
免疫原 EYA4 fusion protein Ag20466 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eyes absent homolog 4 (Drosophila)
别名 CMD1J, DFNA10, EYA4, Eyes absent homolog 4
计算分子量 639 aa, 70 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC041063
基因名称 EYA4
Gene ID (NCBI) 2070
RRIDAB_2879675
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95677
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EYA4 (Eyes absent homolog 4) participates directly in the modulation of cellular signaling through its phosphatase activity and plays a role in the embryonic and post-natal development of the mature organs of CORTI. This protein may be involved in the development of the eye. It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for EYA4 antibody 24691-1-APDownload protocol
IHC protocol for EYA4 antibody 24691-1-APDownload protocol
IF protocol for EYA4 antibody 24691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Res

Lin28a maintains a subset of adult muscle stem cells in an embryonic-like state

Authors - Peng Wang
View Article
humanWB,IHC

Signal Transduct Target Ther

Phosphorylation determines the glucose metabolism reprogramming and tumor-promoting activity of sine oculis homeobox 1

Authors - Yanni Lin
View Article
pigIF

Theriogenology

Analysis of the pluripotent and germline marker gene expression, and the state of X chromosome reactivation of primordial germ cells in pig gonads

Authors - Wenjing Yuan
View Article