MDC1 Polyclonal antibody
MDC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IF | See 3 publications below |
产品信息
24721-1-AP targets MDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | MDC1 fusion protein Ag20135 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mediator of DNA damage checkpoint 1 |
Synonyms | NFBD1, NFBD 1, MDC 1 |
Calculated Molecular Weight | 2089 aa, 227 kDa |
GenBank Accession Number | BC152556 |
Gene Symbol | MDC1 |
Gene ID (NCBI) | 9656 |
RRID | AB_2879688 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14676 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MDC1 antibody 24721-1-AP | Download protocol |
IHC protocol for MDC1 antibody 24721-1-AP | Download protocol |
IF protocol for MDC1 antibody 24721-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination. | ||
Cell Death Dis Wnt signaling induces radioresistance through upregulating HMGB1 in esophageal squamous cell carcinoma. | ||
Cancers (Basel) SFN Enhanced the Radiosensitivity of Cervical Cancer Cells via Activating LATS2 and Blocking Rad51/MDC1 Recruitment to DNA Damage Site. | ||
DNA Repair (Amst) Functional defects of cancer-associated MDC1 mutations in DNA damage repair | ||
Cell Rep The male pachynema-specific protein MAPS drives phase separation in vitro and regulates sex body formation and chromatin behaviors in vivo | ||
Sci Adv VGLL3 modulates chemosensitivity through promoting DNA double-strand break repair |