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验证数据展示

  • WB analysis of HeLa using 24847-1-AP

    WB analysis of HeLa using 24847-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 24847-1-AP

    WB analysis of mouse brain using 24847-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 24847-1-AP

    IHC staining of human kidney using 24847-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 24847-1-AP

    IHC staining of human kidney using 24847-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 24847-1-AP

    IF Staining of HeLa using 24847-1-AP

    Immunofluorescent analysis of HeLa cells using 24847-1-AP (KLHDC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

KLHDC5 Polyclonal antibody

KLHDC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24847-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA1340, Kelch-like protein 42, Kelch domain-containing protein 5, kelch domain containing 5, Cullin-3-binding protein 9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24847-1-AP targets KLHDC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 KLHDC5 fusion protein Ag20448 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch domain containing 5
别名 KIAA1340, Kelch-like protein 42, Kelch domain-containing protein 5, kelch domain containing 5, Cullin-3-binding protein 9
计算分子量 505 aa, 57 kDa
观测分子量 56 kDa
GenBank蛋白编号BC137116
基因名称 KLHDC5
Gene ID (NCBI) 57542
RRIDAB_2879754
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2K6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHDC5 also named as kelch domain containing 5 or KLHL42 is a 505 amino acid protein, which contains one BTB domain and six Kelch repeats. KLHDC5 localizes in cytoplasm and may involve in microtubule dynamics throughout mitosis.

实验方案

Product Specific Protocols
WB protocol for KLHDC5 antibody 24847-1-APDownload protocol
IHC protocol for KLHDC5 antibody 24847-1-APDownload protocol
IF protocol for KLHDC5 antibody 24847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Acta Pharmacol Sin

Satellite cell-derived exosome-mediated delivery of microRNA-23a/27a/26a cluster ameliorates the renal tubulointerstitial fibrosis in mouse diabetic nephropathy

Authors - Jia-Ling Ji
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