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ZNF217 Polyclonal antibody

ZNF217 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24869-1-AP

Print datasheet

Synonyms

ZABC1, ZFP217, zinc finger protein 217, ZNF217

验证数据展示

  • WB analysis using 24869-1-AP

    WB analysis using 24869-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24869-1-AP (ZNF217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of NCCIT using 24869-1-AP

    IP experiment of NCCIT using 24869-1-AP

    IP result of anti-ZNF217 (IP:24869-1-AP, 4ug; Detection:24869-1-AP 1:300) with NCCIT cell lysate 3200ug.

经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, MDA-MB-231 cells
Positive IP detected inNCCIT cell

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24869-1-AP targets ZNF217 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen ZNF217 fusion protein Ag19468 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 217
Synonyms ZABC1, ZFP217, zinc finger protein 217, ZNF217
Calculated Molecular Weight 1048 aa, 115 kDa
Observed Molecular Weight~130 kDa
GenBank Accession NumberBC113427
Gene Symbol ZNF217
Gene ID (NCBI) 7764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF217 antibody 24869-1-APDownload protocol
IP protocol for ZNF217 antibody 24869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols