验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
25148-1-AP targets TBX15 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | TBX15 fusion protein Ag18423 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | T-box 15 |
| 别名 | T box 15, TBX14, TBX15 |
| 计算分子量 | 602 aa, 66 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC122553 |
| 基因名称 | TBX15 |
| Gene ID (NCBI) | 6913 |
| RRID | AB_2879924 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96SF7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBX15 is a transcription factor from the T-box family and is a highly pleiotropic gene expressed in multiple tissues at different stages of development. It is required for skeletal development and deletions in this gene cause severe skeletal malformation. In addition, TBX15 also plays a role in the differentiation of brown and beige adipocytes (PMID: 28007980). The predicted molecular weight of TBX15 is 66 kDa. With modification, the MW of TBX15 will be migrated to 50 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBX15 antibody 25148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |