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验证数据展示

  • WB analysis of NIH/3T3 using 25489-1-AP

    WB analysis of NIH/3T3 using 25489-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25489-1-AP (TMEM199 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25489-1-AP

    IHC staining of human breast cancer using 25489-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 25489-1-AP

    IHC staining of human breast cancer using 25489-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 40x lens).

TMEM199 Polyclonal antibody

TMEM199 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25489-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C17orf32, TMEM199, transmembrane protein 199

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25489-1-AP targets TMEM199 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 TMEM199 fusion protein Ag22009 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 199
别名 C17orf32, TMEM199, transmembrane protein 199
计算分子量 208 aa, 23 kDa
观测分子量 18-23 kDa
GenBank蛋白编号BC033113
基因名称 TMEM199
Gene ID (NCBI) 147007
RRIDAB_2880101
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N511
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM199, also named as C17orf32, is a component of the complex sense antisense architecture (CSAGA).

实验方案

Product Specific Protocols
WB protocol for TMEM199 antibody 25489-1-APDownload protocol
IHC protocol for TMEM199 antibody 25489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2

Authors - Jiming Chen
View Article