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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis of mouse testis using 25518-1-AP

    WB analysis of mouse testis using 25518-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25518-1-AP (PGAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PGAM2-Specific Polyclonal antibody

PGAM2-Specific Polyclonal Antibody for WB, ELISA
Cat No. 25518-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

PGAM2, BPG dependent PGAM 2, BPG-dependent PGAM 2, EC:5.4.2.11, Muscle-specific phosphoglycerate mutase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25518-1-AP targets PGAM2-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoglycerate mutase 2 (muscle)
别名 PGAM2, BPG dependent PGAM 2, BPG-dependent PGAM 2, EC:5.4.2.11, Muscle-specific phosphoglycerate mutase
计算分子量 28 kDa
观测分子量 29 kDa
GenBank蛋白编号NM_000290
基因名称 PGAM2
Gene ID (NCBI) 5224
RRIDAB_2880115
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15259
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody is specific for PGAM2.

实验方案

Product Specific Protocols
WB protocol for PGAM2-Specific antibody 25518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols