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OLIG2 Polyclonal antibody

OLIG2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25754-1-AP

Print datasheet

Synonyms

OLIG 2, Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1

验证数据展示

  • IHC staining of human gliomas using 25754-1-AP

    IHC staining of human gliomas using 25754-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with OLIG2 antibody included
  • WB analysis of rat brain using 25754-1-AP

    WB analysis of rat brain using 25754-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25754-1-AP (OLIG2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IF Staining of mouse brain using 25754-1-AP

    IF Staining of mouse brain using 25754-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (25754-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 25754-1-AP

    IF Staining of mouse brain using 25754-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (25754-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat brain tissue, C6 cells
Positive IHC detected inhuman gliomas tissue, mouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy OLIG2 Ready-To-Use IHC Kit. OLIG2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25754-1-AP targets OLIG2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OLIG2 fusion protein Ag22642 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oligodendrocyte lineage transcription factor 2
Synonyms OLIG 2, Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC047511
Gene Symbol OLIG2
Gene ID (NCBI) 10215
RRIDAB_2880226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13516
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.

实验方案

Product Specific Protocols
WB protocol for OLIG2 antibody 25754-1-APDownload protocol
IHC protocol for OLIG2 antibody 25754-1-APDownload protocol
IF protocol for OLIG2 antibody 25754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols