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验证数据展示

  • WB analysis of HepG2 using 25872-1-AP

    WB analysis of HepG2 using 25872-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25872-1-AP (LY6G6F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 25872-1-AP

    WB analysis of HL-60 using 25872-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25872-1-AP (LY6G6F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

LY6G6F Polyclonal antibody

LY6G6F Polyclonal Antibody for WB, ELISA
Cat No. 25872-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, ELISA and More (1)

别名

C6orf21, G6f, LY6G6D, LY6G6F, NG32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HL-60 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

25872-1-AP targets LY6G6F in WB, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性mouse
免疫原 LY6G6F fusion protein Ag22467 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte antigen 6 complex, locus G6F
别名 C6orf21, G6f, LY6G6D, LY6G6F, NG32
计算分子量 297 aa, 32 kDa
观测分子量 60 kDa
GenBank蛋白编号BC137212
基因名称 LY6G6F
Gene ID (NCBI) 259215
RRIDAB_2880278
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SQ64
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY6G6F, also named as C6orf21, G6F, LY6G6D and NG32, plays a role in the downstream signal transduction pathways involving GRB2 and GRB7. It has monomer (~30 kda) and homodimer (~60 kDa).

实验方案

Product Specific Protocols
WB protocol for LY6G6F antibody 25872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Arterioscler Thromb Vasc Biol

Caspase-8 Promotes Pulmonary Hypertension by Activating Macrophage-Associated Inflammation and IL-1β (Interleukin 1β) Production.

Authors - Wuwei Rong
View Article