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TSEN15 Polyclonal antibody

TSEN15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26054-1-AP

Print datasheet

Synonyms

tRNA intron endonuclease Sen15, SEN15 homolog, sen15, HsSEN15, C1orf19

验证数据展示

  • WB analysis using 26054-1-AP

    WB analysis using 26054-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26054-1-AP (TSEN15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, human testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26054-1-AP targets TSEN15 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TSEN15 fusion protein Ag22082 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tRNA splicing endonuclease 15 homolog (S. cerevisiae)
Synonyms tRNA intron endonuclease Sen15, SEN15 homolog, sen15, HsSEN15, C1orf19
Calculated Molecular Weight 171 aa, 19 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC022030
Gene Symbol TSEN15
Gene ID (NCBI) 116461
RRIDAB_3669520
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSEN15 is the non-catalytic subunit of the tRNA-splicing endonuclease complex, which is a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini (PMID: 15109492, 27392077).

实验方案

Product Specific Protocols
WB protocol for TSEN15 antibody 26054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols