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验证数据展示

  • WB analysis using 26678-1-AP

    WB analysis using 26678-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26678-1-AP (INPPL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 26678-1-AP

    IP experiment of HeLa using 26678-1-AP

    IP result of anti-INPPL1 (IP:26678-1-AP, 4ug; Detection:26678-1-AP 1:800) with HeLa cells lysate 2520 ug.

  • IHC staining of mouse stomach using 26678-1-AP

    IHC staining of mouse stomach using 26678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26678-1-AP (INPPL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with INPPL1 antibody included

INPPL1 Polyclonal antibody

INPPL1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 26678-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

INPPL 1, INPPL1, Protein 51C, SHIP 2, SHIP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy INPPL1 Ready-To-Use IHC Kit. INPPL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26678-1-AP targets INPPL1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 INPPL1 fusion protein Ag24790 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate phosphatase-like 1
别名 INPPL 1, INPPL1, Protein 51C, SHIP 2, SHIP2
计算分子量 1258 aa, 139 kDa
观测分子量150 kDa
GenBank蛋白编号BC140853
基因名称 INPPL1
Gene ID (NCBI) 3636
RRIDAB_3085891
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15357
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for INPPL1 antibody 26678-1-APDownload protocol
IHC protocol for INPPL1 antibody 26678-1-APDownload protocol
IP protocol for INPPL1 antibody 26678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols