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DUSP9 Polyclonal antibody

DUSP9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26718-1-AP

Print datasheet

Synonyms

MKP4, MKP 4, Mitogen-activated protein kinase phosphatase 4, MAP kinase phosphatase 4, EC:3.1.3.16

验证数据展示

  • WB analysis using 26718-1-AP

    WB analysis using 26718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26718-1-AP (DUSP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 26718-1-AP

    IHC staining of human kidney using 26718-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 26718-1-AP

    IHC staining of human kidney using 26718-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26718-1-AP (DUSP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26718-1-AP targets DUSP9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen DUSP9 fusion protein Ag25167 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dual specificity phosphatase 9
Synonyms MKP4, MKP 4, Mitogen-activated protein kinase phosphatase 4, MAP kinase phosphatase 4, EC:3.1.3.16
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC060837
Gene Symbol DUSP9
Gene ID (NCBI) 1852
RRIDAB_2880612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DUSP9 antibody 26718-1-APDownload protocol
IHC protocol for DUSP9 antibody 26718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

Identifying specific TLS-associated genes as potential biomarkers for predicting prognosis and evaluating the efficacy of immunotherapy in soft tissue sarcoma

Authors - Xiang-Xu Wang
View Article
mouseWB

Sci Rep

Inhibition of miR-194-5p avoids DUSP9 downregulation thus limiting sepsis-induced cardiomyopathy

Authors - Jie Wang
  • KD Validated
View Article